Table 1.
Polypeptide fragments of NaeI produced by limited proteolysis
| Fragment* | Sequence of N terminus† | Sequence position of polypeptide fragments‡ |
|---|---|---|
| T19.1 | LTPEGRA | 151 |
| T17.3 | MTELPLQ | 1 |
| T16.6 | MTELPLQ | 1 |
| C19.1 | LTPEGRA | 151 |
| C13.5 | QFAEPDD | 7 |
*
Fragments are named according to protease treatment (T, trypsin; C, chymotrypsin) and apparent molecular size of fragment (in kDa) based on relative mobility during SDS/PAGE (e.g., T19.1 = fragment of approximately 19.1 kDa produced by trypsin digestion).
†
Sequences shown were determined by automated amino acid sequencing of proteolytic reaction products as described in Materials and Methods.
‡
The sequence position of each polypeptide fragment is given as the position of the first amino acid residue of the N-terminal end of the fragment relative to the amino acid sequence of NaeI.