Figure 3.

Protein splicing by reconstitution of fusion proteins containing N- and C-terminal fragments of the Mtu RecA intein. (A) Control experiment. Western blot analysis of MU_NΔ and U_CΔH (lane 2), MU_NΔ alone (lane 3), and U_CΔH alone (lane 4) carried through the renaturation and protein splicing protocols described in Materials and Methods. (B) Time course. Western blot analysis of reconstituted mixtures of MU_NΔ and U_CΔH allowed to undergo protein splicing for the times indicated. Western blot analysis was done as described in Materials and Methods using anti-His-tag antibody, which selectively reveal the unspliced precursor, U_CΔH, and the spliced product, MH.