Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of temperature on protein splicing. Mixtures of MUNΔ and UCΔH were reconstituted under standard conditions at 4°C, supplemented with 1.25 mM DTT to induce splicing and incubated for 6 hr at the temperatures indicated. Protein splicing was assayed by Western blot analysis with anti-His-tag antibody, and the relative amounts of MH were estimated by densitometry of the Western blot.
