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. 1998 Mar 31;95(7):3549–3554. doi: 10.1073/pnas.95.7.3549

Figure 2.

Figure 2

Western blots of PDE4A. 3T3-F442A cells were subjected to SDS/PAGE and subsequent Western blotting with a PDE4A-specific antiserum. In A cells were untreated (lane a) or treated with GH (25 nM) alone (lane b), GH + wortmannin (50 nM; lane c), GH + LY294002 (2 μM; lane d), GH + rapamycin (10 nM; lane e), and GH + actinomycin D (4 μg/ml; lane f). In control cells a single immunoreactive species of ≈108 kDa was observed whereas in cells where treatment with GH caused PDE4 activation a species of lower mobility was observed (A, tracks b and f) of size ≈109 kDa. In B cells were either untreated (lane a) or treated with antisense (10 nM) to PDE4A5 (lane b). These data are typical of experiments done three times.