Table 1.

Summary of the proteins identified to be increasingly carbonylated in synaptosomes treated with acrolein (0.5 μM) for 30 min.

Protein	Mowse score	Peptides matched	% Coverage	MW (kD)	pI	Oxidation (fold-increase)	P value
Tropomyosin 3 gamma isoform 2	95	10	26	29,2	4.75	2.3 ± 1.3	P=0.024
Tropomyosin 5	95	9	32	29,1	4.72	7.3 ± 3.8	P=0.011
Beta-actin	85	10	32	39,4	5.78	8.1 ± 6.2	P=0.017
Tu translation elongation factor	92	8	20	49,8	7.23	11.0 ± 2.1	P=0.025
VDAC-1 – outer mitocondrial porin 1	69	7	34	30,6	8.63	13.0 ± 4.3	P=0.047

Proteins were trypsinized and analyzed by Mass Spectrometry in order to ascertain their identities. Proteins with a Mowse score >66 were considered to be identified at a statistically significant level. MW, molecular weight; pI, isoelectric point. Statistical analysis was carried out by the one-tailed paired T-test.