Table 1.

Frequency of excision of the FRT-hyg-FRT cassette by Flp recombinase encoded by flp_e and flp_m.

Gene	Strain	Number of clones	Excision frequency	References
flpe	M. smegmatis	40	5%	(Stephan et al., 2004)
	M. smegmatis	40	10%	(Stephan et al., 2004)
	M. smegmatis	200	0.5%	This study
	M. bovis BCG	ND	0%	(Stephan et al., 2004)
	M. bovis BCG	200	0%	This study
flpm	M. smegmatis	200	64%	This study
	M. smegmatis	120	63%	This study
	M. bovis BCG	200	40%	This study
	M. bovis BCG	32	59%	This study

ND: not determined