Table 1.
Rates of oxidation of olefinic substrates by P450 cytochromes
| Substrate | Product determined | Reaction rate, nmol/min per nmol of P450
|
|||
|---|---|---|---|---|---|
| Δ2B4 | Δ2B4 T302A | Δ2E1 | Δ2E1 T303A | ||
| Styrene | Styrene oxide | 47.8 ± 0.5 | 15.9 ± 0.8 | 14.5 ± 0.6 | 33.0 ± 0.4 |
| Cyclohexene | Cyclohexene oxide | 48.7 ± 4.4 | 13.0 ± 0.5 | 6.7 ± 0.1 | 11.4 ± 0.2 |
| Cyclohexene | 1-cyclohexene-3-ol | 29.3 ± 1.7 | 7.8 ± 1.1 | 6.6 ± 0.7 | 4.8 ± 0.1 |
| cis-2-butene | cis-2-butene oxide | 6.4 ± 0.6 | 3.8 ± 0.1 | 36.0 ± 2.0 | 97.0 ± 3 |
| cis-2-butene | 2-butene-1-ol | 0.15 ± 0.02 | <0.05* | 0.40 ± 0.03 | 0.21 ± 0.06 |
| trans-2-butene | trans-2-butene oxide | 3.8 ± 0.2 | 0.48 ± 0.03 | 4.8 ± 0.1 | 27.8 ± 0.5 |
| trans-2-butene | 2-butene-1-ol | 0.11 ± 0.02 | <0.05* | 1.2 ± 0.1 | 0.5 ± 0.1 |
The results are given as the mean with the SD for triplicate determinations from a typical experiment.
*
Only a trace amount of the alcohol was formed by the mutant Δ2B4 enzyme.