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Effect of Thr → Ala mutation on the ratio of the rates of epoxidation and hydroxylation of olefins
| Substrate | Ratio of initial rates, epoxidation/hydroxylation
|
|||
|---|---|---|---|---|
| Δ2B4 | Δ2B4 T302A | Δ2E1 | Δ2E1 T303A | |
| Cyclohexene | 1.7 | 1.7 | 1.0 | 2.4 |
| cis-butene | 43 | ND* | 90 | 460 |
| trans-butene | 35 | ND* | 4.0 | 56 |
ND, not determined (only a trace of the alcohol was formed by this enzyme).
