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Interstem angles from previous single-bend measurements
| Element | Interstem angle, degrees
|
Ref. | |
|---|---|---|---|
| Mg2+ (2 mM) | No Mg2+ | ||
| E[tRNAPhe] | 70 ± 4 | 154 ± 5 | 17 |
| A5 bulge | 106 ± 3 | 105 ± 3 | 19 |
Interstem angles subtend the axes of the helices flanking the bend element; they are thus supplements to the previously reported bend angles. For E[tRNAPhe], the angle subtends the extended acceptor and anticodon stems.
