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. Author manuscript; available in PMC: 2007 Sep 20.
Published in final edited form as: Virology. 2007 Jan 31;362(2):283–293. doi: 10.1016/j.virol.2006.12.015

Figure 1. MCH Genetic strategy.

Figure 1

(A) MCH screen. Entire pool was first transduced at low MOI with HIV-CIB(VSV G), selected using 10 μg/ml blasticidin, and then broken down into smaller pools of ∼25 clones. Each smaller pool was transfected with VSV G expression plasmid and rescued HIV titered on naive human cells. Positive pools were broken down to single cells and the process iterated to identify permissive clones (B) Schematic of pHIV-CIB. Truncated cyclin T1 is expressed as part of a bicistronic cassette along with Blasti, blasticidin deaminase. LTR denotes long terminal repeat, G gag, P pol, T tat, R rev, RRE Rev response element, ψ packaging signal. Gene products not to scale.