Skip to main content
. Author manuscript; available in PMC: 2007 Sep 20.
Published in final edited form as: Virology. 2007 Jan 31;362(2):283–293. doi: 10.1016/j.virol.2006.12.015

Figure 8. Effects of synthetic gag-pol on HIV release.

Figure 8

(A) Parental B78 cells were transiently transfected with codon-optimized Gag-eGFP and then subjected to deconvolution microscopy 3 days later. (B, C) MCHs stably transduced with HIV-CIB and 293T cells transduced with HIV-LIB were transiently transfected with HIV-eYFP and pME-VSV G, with or without the Oxford Biomedica synthetic Gag-Pol (SGP) plasmid. (B) Supernatant (upper panel) and cell lysate (lower) were subjected to SDS-PAGE and immunoblotting as per figure 5. Lane 1: parental B78s; 2: B78s+SGP; 3: p11; 4: p11+SGP; 5: p11-R; 6: p11-R+SGP; 7: p28; 8: p28+SGP; 9: 293Ts. Short arrows indicate CA, * MA. (C) Supernatant harvested at 48 hours was titered on naïve human targets. Titer was normalized to transfection efficiency, as judged by epifluorescence microscopy of the producers. Open bars: eYFP titer; closed bars BlastiR titer. R denotes revertant. This experiment was repeated, with similar results.