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. Author manuscript; available in PMC: 2008 Mar 13.
Published in final edited form as: Cancer Cell. 2007 Mar;11(3):275–289. doi: 10.1016/j.ccr.2007.01.014

Figure 2. G1/S and S/G2 transitions are not affected by Lzts1 absence in MEFs.

Figure 2

A. Growth curve comparing of Lzts1+/+ (black square) and Lzts1−/− (gray square) cells over 6 days. B. FACS analysis of DNA content of Lzts1+/+ and Lzts1−/− MEFs in logarithmic phase of growth. C. Cell cycle distribution of Lzts1+/+ and Lzts1−/− MEFs serum-starved for 24 hours (starved) and then re-stimulated with 10% FBS containing-medium (FCS) for the indicated time. D. Western blot analysis of cell cycle related proteins in Lzts1+/+ and Lzts1−/− cells treated as in C. E. Western blot analysis of Geminin expression in Lzts1+/+ and Lzts1−/− MEFs serum-starved for 24 hours (starved) and then re-stimulated with 10% FBS containing-medium for the indicated time. In the lower graph the expression of Geminin normalized on the levels of vinculin at each time point is reported.