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Construction of singly modified genomes as described. Briefly, a singly modified heptamer was ligated into the lacZ coding region of an M13mp7L2 bacteriophage genome. The three bases on the 3′ end of the M13 molecule combined with the three bases on the 5′ end of the oligonucleotide generate a unique recognition site for the restriction enzyme ApaLI, 5′-GTGCAC-3′.
