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Melting temperature as a function of pH for the mixture of oligonucleotides (1 μM each), which can form either a 14-mer parallel Hoogsteen duplex in the presence of 15 μM BfPQ (1), or a 10-bp antiparallel Watson–Crick duplex in the absence of BfPQ (2). Experiments were carried out in a cacodylate buffer (10 mM) containing 0.1 M NaCl.
