Table 1.
Melting temperatures of the triplex and the duplex (sequences shown at the top of Fig. 4) in the absence and presence of 10 μM BgPI, BfPQ, and BQQ molecules
| Ligands | Tm, ±1°C
|
ΔTm, °C
|
||
|---|---|---|---|---|
| Triplex Tm3→2 | Duplex Tm2→1 | Triplex ΔTm3→2 | Duplex ΔTm2→1 | |
| None | 18 | 58 | ||
| BgPI | 46 | 74 | +28 | +16 |
| BfPQ | 47 | 60 | +29 | +2 |
| BQQ | 69 | 62 | +51 | +4 |
Tm3→2 is the melting temperature for the dissociation of triplex into duplex + thrid strand, except for the result obtained in the presence of BQQ, in which the triplex dissociates directly into three single strands. ΔTm indicates the difference of Tm in the presence and absence of ligands. Δ(ΔTm) shows the difference of ΔTm for the 14-mer triplex and the 26-bp duplex. Experiments were carried out in a 10 mM cacodylate buffer (pH 6.2) containing 0.1 M NaCl.