Figure 1.

ODF is an OPG/OCIF-binding protein. (A) Schematic structure of ODF. Domains: C, cytoplasmic; TM, transmembrane; E, extracellular. Arrowhead represents the N terminus (Asp⁷⁶) of ODF, which is fused to the C-terminal end of thioredoxin. (B) Binding analysis of [¹²⁵I]OPG/OCIF to COS-7 cells transfected with the ODF expression vector (COS^ODF) or COS-7 cells transfected with the empty vector (COS^Vec). Data are expressed as mean ± SD of three cultures. (C) Coimmunoprecipitation of ODF-OPG/OCIF complexes with anti-OPG/OCIF antibody (Ab).