Figure 2.

sODF induces OCL formation from spleen cells in the absence of osteoblasts/stromal cells. (A-C) Mouse spleen cells were cultured in the presence of 10 ng/ml of M-CSF and various concentrations of sODF for 6 days. (A) Morphology of OCLs induced from spleen cells in the presence or absence of 30 ng/ml sODF. (B) The number of TRAP-positive multinucleated cells induced by sODF. Data are expressed as mean ± SD of three cultures. (C) Autoradiography of [¹²⁵I]calcitonin bound to OCLs induced by 30 ng/ml of sODF. The cells were incubated with [¹²⁵I]calcitonin, stained for TRAP (Left), and processed for autoradiography (Right). (D) Resorption pits formed on a dentine slice by the induced OCLs. [Bars = 200 μm (A, C, and D).]