Figure 1.

In situ expression of VEGF protein in inoculates of DLD-1- and HCT-116-derived sublines 4 days after tumor cell injection. Clones were designated as AS for antisense VEGF transfectants, S for sense, and V for vector control transfectants respectively. (A) Parental DLD-1 cells. (B) DLD-V1. (C) DLD-AS2. (D) DLD-AS6. (E) Parental HCT-116 cells. (F) HCT-V1. (G) HCT-AS9. (H) HCT-AS18. (I) Mutant K-ras knockout-derived VEGF sense clone DKs-S1. (J) DKs-S6. (K) DKs-S10. (L) HKh-S3. Clones DLD-AS3 and HCT-AS22 ressembled their AS transfected sister cell lines (data not shown).