Table 2.
Characteristics of antisense and sense VEGF transfected colorectal cancer cell lines
| Cell line |
In vitro
|
In vivo
|
||||
|---|---|---|---|---|---|---|
| Doubling time, h | Plating efficiency, % | Colony formation in soft agar, % | No. mice with tumor/no. mice injected | Tumor volume, mm3 | Days after injection | |
| DLD-1 | 33.6 | 40.2 | 15.6 | 6/6 | 1,074 ± 94 | 23 |
| DLD-V1 | 38.4 | 43.6 | 15.3 | 6/6 | 980 ± 41 | 23 |
| DLD-AS2 | 36.0 | 50.8 | 4.6 | 3/4 | 134 ± 132 | 31 |
| DLD-AS2tum+ | 28.8 | 54.2 | 3.5 | N/A | N/A | N/A |
| DLD-AS5 | 52.8 | 40.6 | 14.9 | 2/8 | 9 ± 13 | 45 |
| DLD-AS6 | 76.8 | 53.0 | 8.4 | 0/8 | 7 ± 6 | 45 |
| DLD-AS9 | 19.2 | 54.8 | 26.4 | 2/8 | 15 ± 27 | 45 |
| DLD-AS9tum+ | 16.8 | 74.6 | 25.2 | N/A | N/A | N/A |
| HCT-116 | 57.6 | 56.6 | 18.6 | 6/6 | 466 ± 229 | 31 |
| HCT-V1 | 62.4 | 52.8 | 18.8 | 6/6 | 456 ± 215 | 31 |
| HCT-AS9 | 57.6 | 51.2 | 17.3 | 0/8 | 0 | 45 |
| HCT-AS18 | 55.2 | 51.4 | 18.3 | 6/8 | 134 ± 208 | 45 |
| HCT-AS18tum+ | 48.0 | 40.8 | 20.7 | N/A | N/A | N/A |
| HCT-AS22 | 81.6 | 38.0 | 11.2 | 3/8 | 64 ± 76 | 45 |
| HCT-AS22tum+ | 38.4 | 56.6 | 13.3 | N/A | N/A | N/A |
| DKs-8 | 57.6 | 54.8 | 0 | 0/12 | 0 | 160 |
| DKs-V1 | 57.6 | 56.0 | 0 | 0/12 | 0 | 160 |
| DKs-S1 | 100.8 | 30.8 | 0 | 2/10 | 59 ± 154 | 35 |
| DKs-S6 | 40.8 | 14.2 | 0 | 2/16 | 43 ± 145 | 160 |
| DKs-S10 | 26.4 | 22.0 | 0 | 7/16 | 109 ± 132 | 69 |
| DKs-S10tum+ | 48.0 | 34.6 | 0 | N/A | N/A | N/A |
| HKh-2 | 48.0 | 34.6 | 0 | 0/10 | 0 | 160 |
| HKh-V1 | 50.4 | 40.0 | 0 | 0/8 | 0 | 160 |
| HKh-S3 | 64.8 | 21.8 | 0 | 1/18 | 17 ± 72 | 160 |
| HKh-S8 | 57.6 | 23.4 | 0 | 5/14 | 51 ± 4 | 160 |
To determine the ability of tumors to form in vivo, approximately 1 × 106 DLD-1, HCT-116, and their transfected cell lines or 1 × 107 DKs-8, HKh-2, and their transfected cell lines were injected subcutaneously into BALB/c nude mice and the number of mice forming tumors was recorded. Results are from two experiments with similar results. Data for tumor volume are the mean ± SD. N/A, not applicable.