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MMP9 and MMP2 activity in EBV-negative cells transfected with EBV oncoprotein LMP1 or EBNA2. (Top) Gelatinolytic activity of C33A cells transfected with LMP1 and EBNA2. (Middle and Bottom) Immunoblots for LMP1 and EBNA2 protein. Cells (4.0 × 105) were placed in three sets of 60-mm culture plates 12 hr before transfection. Samples were prepared for zymography, and LMP1 and EBNA2 protein detection.
