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. 2001 Jan 15;107(2):163–171. doi: 10.1172/JCI10310

Figure 1.

Figure 1

Reconstitution of recipient mice with donor marrow. (a) ACAT1 genotyping in recipients of fetal liver cell transplants. DNA was prepared from the bone marrow of LDLR–/– recipient mice at least 4 weeks after transplantation. The genomic DNA was amplified in a PCR reaction. The absence of the wild-type band in recipients of ACAT1–/– cells indicates complete repopulation of the marrow with cells of donor origin. (b and c) Immunocytochemical analyses of the proximal aorta. Cryosections (5 μm) were treated according to our previously published method (46) and stained for ACAT1 using a rabbit antibody to recombinant human ACAT1 (31).