Figure 2.

Chemotactic responses of resident DCs, migrated cutaneous DCs, and B cells to CXCL12. A and B: Draining lymph node cells were prepared from mice 24 hours after FITC application on the abdomen. Input cells and cells that migrated to the lower well of a transwell chamber in the absence of CXCL12 (0) or in response to 3, 30, or 300 ng/ml CXCL12 were analyzed by flow cytometry to detect MHC class II⁺ FITC⁺-migrated cutaneous DCs, MHC class II⁺ FITC⁻ resident DCs (A), and B220⁺ B cells (B). Filled symbols indicate three independent experiments, and columns represent the average. A Student’s t-test was performed between the indicated groups, and an asterisk indicates P < 0.05.