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. 2007 Oct;171(4):1249–1257. doi: 10.2353/ajpath.2007.070225

Figure 6.

Figure 6

Perturbed initiation of skin immune response by blockade of CXCR4 engagement. A: Effect of CXCR4 antagonist on CHS response to DNFB. The ear thickness of DNFB-sensitized mice treated with either vehicle alone (−) or CXCR4 antagonist during sensitization (S), elicitation (E), or both (S+E) was measured after challenge with DNFB. *P < 0.05 versus vehicle-treated mice (n = 6 per group). B: H&E staining of ears from mice treated with (CXCR4 ant+) or without (CXCR4 ant-) CXCR4 antagonist 24 hours after challenge with DNFB. Scale bar = 100 μm. C: The proliferative response of DNFB-immune lymphocytes to DNBS. The lymph node cells from mice sensitized by DNFB with or without CXCR4 antagonist (CXCR4 ant) treatment were stimulated with DNBS for 72 hours. Lymph node cells eliminated by CD11c+ DCs were also prepared using autoMACS. The proliferative response was measured in triplicate. Columns show the mean ± SD from triplicate wells. Student’s t-test was performed between the indicated groups, and an asterisk indicates P < 0.05. Results are representative of three independent experiments. cpm, counts per minute.