Skip to main content
. 2007 Oct;171(4):1281–1290. doi: 10.2353/ajpath.2007.070472

Figure 8.

Figure 8

Role of tPA and LRP on cerebral ischemia-induced iNOS expression. A: Representative picture of immunohistochemical analysis of iNOS expression in the area of ischemic penumbra (AOI-2) in WT (a and b) and tPA−/− (c and d) mice 24 hours after MCAO. Green is iNOS, and blue is DAPI. NC, necrotic core. Each observation was repeated four times. B: Quantitative real-time PCR (RT-PCR) analysis of iNOS expression in brain extracts from WT (black bars), tPA−/− (white bars), and WT mice treated with the RAP (gray bars) 0.1 to 72 hours after MCAO. Error bars described SEM. n = 7 for each observation. *P < 0.05 when compared with treated and untreated WT animals sacrificed at each time point after MCAO. †P < 0.05 when compared with untreated WT mice at each time point. C: RT-PCR analysis of iNOS expression in primary murine astrocytic cultures exposed to OGD conditions for 4 hours and incubation with either PBS (black bars) or PBS and neuroserpin (NS, white bars). n = 4 for each observation. *P < 0.05 when compared with NS-treated cells. Original magnifications, ×40.