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. 1998 Mar 31;95(7):3644–3649. doi: 10.1073/pnas.95.7.3644

Figure 5.

Figure 5

Etk is involved in the IL-6-induced neuroendocrine differentiation of LNCaP. (A–C) IL-6 induces morphological changes of LNCaP. (A) LNCaP cells in the serum-free medium (×400 magnification); (B) LNCaP cells treated with 50 ng/l IL-6 for 24 hr (×400 magnification); (C) LNCaP cells treated with 50 ng/ml IL-6 for 72 hr (×100 magnification). (D, E) Overexpression of the dominant negative Etk (EtkDN) in LNCaP blocks the IL-6-induced morphological changes. LNCaP cells were transfected with pcDNA3-T7-EtkDN and selected in the presence of G418, resulting in LNCaP cells overexpressing EtkDN (LN-EDN). (D) LN-EDN in serum-free medium; (E) LN-EDN treated with 50 ng/ml IL-6 for 24 hr. (F) IL-6 induces the enhanced expression of NSE in LNCaP, but not in LN-EDN. 50 μg of the cell extracts from the IL-6-treated (+) or untreated (−) LNCaP or LN-EDN were resolved on an SDS/7.5% polyacrylamide gel and followed by Western blot analysis using antibody specific for NSE.