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. Author manuscript; available in PMC: 2007 Sep 21.
Published in final edited form as: Traffic. 2007 Feb 7;8(4):347–358. doi: 10.1111/j.1600-0854.2007.00534.x

Figure 4.

Figure 4

To determine whether karyopherins with defects in actin organisation and endocytosis were also affected in their localisation of Sla1p, Sla1p was tagged in three strains and localised as described in materials and methods. As shown, in the srp1-31 and rsl1-4 strains (A,B) Sla1p cannot be observed in the nucleus. In the crm1T539C mutant in the presence of the inhibitor leptomycinB, (LMB at 100 ng/ml for 2 hours). Sla1p can be observed both in the nucleus and at the cell cortex indicating that Crm1 is not the main exportin for Sla1p. Bars = 5 μm.