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. Author manuscript; available in PMC: 2007 Sep 21.
Published in final edited form as: Pharmacogenet Genomics. 2007 Jan;17(1):37–45. doi: 10.1097/01.fpc.0000236325.73186.2c

Figure 1.

Figure 1

Effect of individual SNPs and combinations (alleles) on catalytic activity, immunoreactive protein level, and the steady state mRNA level in COS-1 cells. (A): SMZ NAT2 activities in COS-1 cells expressing NAT2 alleles with different SNPs. Each bar represents mean ± SD of at least three independent transfections. NAT2 activities for NAT2 12D, NAT2 5I and NAT2-364A were below the limit of detection (0.3 nmol/min/mg). *Significantly lower than NAT2 4 (Ref), p<0.01. (B): Western blot of COS-1 cells expressing different NAT2 alleles as the order showed in (A). Cell lysates (20 μg total protein) were separated on 12% Tris-Glycine PAGE gel and then blotted on nitrocellulose membrane. The blots were probed with anti-NAT2 or anti-α-tubulin primary antibody. (C): Relative NAT2 mRNA levels in transfected COS-1 cells. The NAT2 mRNA was first normalized to internal β-actin and then expressed relative to the NAT2 mRNA level in NAT2*4 transfected COS-1 cells (defined as 1.0). Each bar shows the average and S.D. of the relative level based on three independent transfections. None of the mRNA levels differed significantly (p>0.05) from NAT2*4 (Ref).