Figure 3.

Western blot transfected cells were suspended in lysis buffer. Following one cycle of freeze-thawing, the suspension was supplemented with 1% (w/w) of Triton X-100 and incubated for 30 min on ice. After a 60 min centrifugation (15,000 × g at 4 °C), the supernatant was isolated from the pellet, which was subsequently redissolved in 2% SDS-containing lysis buffer and sonicated for 10 seconds. Both the supernatant and the redissolved pellet were subjected to western blot as described above. Ref: NAT2 4.