dNox regulates proctolin-induced calcium influx in ovarian muscle. (A–B) Aequorin luminescence after addition of 1 μM proctolin and 1 μM ionomycin. Control, UAS-DN352-IR; dNox RNAi, UAS-DN352 + TubP-GAL4. (A) Squares, control ovaries; triangles, dNox RNAi ovaries. (B) Black, control ovaries; red, control ovaries + 10 μM DPI; yellow, control ovaries + 20 μM DPI; green, control ovaries + 40 μM DPI. (B inset) Quantification of data from (A) and (B); n=5 for all samples and error bars represent standard deviation, n=5. (C) Circles, control ovaries + 1 μM proctolin; squares, control ovaries + 100 μM hydrogen peroxide; triangles, control ovaries + 1 μM proctolin + 100 μM hydrogen peroxide. (D) Schematic of mechanism for dNox regulation of Drosophila ovarian function.