FIGURE 2. Lineweaver-Burk plots for procainamide inhibition of DNMT1.
DNMT1 concentration was fixed at 100 nm for hemimethylated DNA and 200 nm for unmethylated DNA. A, procainamide inhibition of DNMT1 on hemimethylated DNA with AdoMet concentration fixed at 1 μm. Procainamide concentration was fixed at 0 μm (filled circles), 100 μm (open circles), 500 μm (filled triangles), 2 mm(open triangles), and 8 mm (filled squares). B, procainamide inhibition of DNMT1 on hemimethylated DNA with CG concentration fixed at 1 μm. Procainamide concentration was fixed at 0 μm (filled circles), 100 μm (open circles), 500 μm (filled triangles), 2 mm (open triangles), and 8 mm (filled squares). C, procainamide inhibition of DNMT1 on unmethylated DNA with AdoMet concentration fixed at 1 μm. Procainamide concentration was fixed at 0 mm (filled circles), 2 mm (open circles), 4 mm (filled triangles), and 8 mm (open triangles). D, procainamide inhibition of DNMT1 on unmethylated DNA with CG concentration fixed at 1 μm. Procainamide concentration was fixed at 0 mm (filled circles), 2 mm (open circles), 4 mm (filled triangles), and 8 mm (open triangles).