Fig. 3.

Light micrograph (kindly provided by Dr. Attila Gulyas) shows the result of a combined anterograde tracing and calretinin immunostaining study, in the dentate gyrus. The anterograde tracer, biotinylated dextran amine (BDA) was injected into the medial septum diagonal band. Large boutons of BDA-containing axons form multiple, basket-like, putative synaptic contacts with the soma of calretinin-immunoreactive (labeled with a brown diaminobenzidine reaction product) neurons. One of these cells (arrows) located in the supragranular layer (SgL) the other is at the border between the granule cell layer (GcL) and dentate hilar area (H).

Bar scale= 50 μm.