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Temperature dependence of absorbance of the holo-protein of TtCuA at 530 nm (ɛ530nm) at pH 6.5 (□) and pH 4 (▪) in absence of any denaturant and in presence of 4 M (pH 6.5, ▵) GuHCl and 2 M (pH 4, ▴) GuHCl.