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Variation of free energy parameters of unfolding with pH for holo-protein of TtCuA containing the native mixed-valence state of copper center (•), holo-protein of TtCuA containing the reduced state of copper center (▪) and apo-protein of TtCuA (▴) at 20°C. The value at pH 7 for the native holo-protein was taken from Wittung-Stafshede et al. (15).
