TABLE 2.
Statistics for the Fn/peptide system
qN% | Tip | F (pN) |
---|---|---|
q0.6% | Bare | 21.4 (6.2) |
Peptide | 22.5 (9.8) | |
q2.0% | Bare | 34.4 (10.2) |
Peptide | 54.8 (22.3) | |
q3.0% | Bare | 55.7 (24.2) |
Peptide | n.a. |
When adsorbed Fn is present, there is a high probability that an interaction with the peptide-functionalized tip will occur. The method used to calculate interactions does not consider the point of separation as an interaction. For both the bare tip and the collagen-related-peptide-functionalized tip, the force increases with surface concentration of Fn. The highest surface concentration used with the collagen-related-peptide-functionalized tip resulted in significant tip fouling, and an interaction force was not calculated. The values were calculated from the empirical probability density function, where μ = Σxipi and σ2 = Σ(xi − μ)2pi. Standard deviations are in parentheses. n.a., not applicable.