Table 3.
Activation and repression of the vertex regulatory genes
| Flies | engrailed, % (n) | hedgehog, % (n) | wingless, % (n) | orthodenticle,* % (n) |
|---|---|---|---|---|
| Wild type | 100 (9) | 100 (15) | 100 (6) | 100 (11) |
| oc1/Y; +/+ | 0 (28) | 0 (27) | 17 (23) | 0 (16) |
| oc1/Y; hsp-otd/+ | ||||
| line 5A | 73 (19) | 71 (28) | 100 (19) | 13 (16) |
| oc1/Y; hsp-Otx1/+ | ||||
| line 3.19.1 | 27 (15) | 52 (23) | 70 (23) | 0 (18) |
| oc1/Y; hsp-Otx2/+ | ||||
| line 8.13.6 | 56 (27) | 19 (27) | 65 (20) | 0 (12) |
| oc1/Y; hsp-ems/+ | ||||
| line 13-1 | 0 (19) | 0 (12) | 8 (12) | 0 (17) |
Numbers of examined discs are shown in parentheses. Discs with positive induction in the vertex primordium were counted for engrailed and hedgehog. Discs showing suppression in the vertex primordium were counted for wingless. Heat pulses were applied four times between 72 and 85 h after egg laying.
*
Signals were detected with a specific RNA probe for the fly otd sequence. Residual otd expression was present in oc1 discs. Transformant discs with similar basal expression were not counted. Broad staining over the eye–antennal discs was observed for hsp-otd discs because of the ubiquitous induction of the otd gene by the heat shock promoter.