Figure 2.

Southern blot analysis of G₂ genomic DNA from the transformed families, digested with SacI and hybridized with the 4.7-kb cn⁺ gene fragment. The predicted 2.5-kb internal fragment is observed in each case, as is a flanking fragment of greater than 3.0 kb, representing a single complete insertion event. Family 128 contains an additional 1.4-kb hybridizing fragment.