Figure 1.

Strategy for identifying sequences after a genetic selection. Rather than individual purification and dideoxy sequencing, all clones are pooled from plates and plasmid DNA is isolated in a single purification. PCR amplification using primers with 3′ sequence corresponding to vector sequence is used to selectively enrich for insert DNA from the plasmid pool. Amplified insert DNA is fragmented with DNase I, labeled with biotin-ddATP, and hybridized to an array containing oligonucleotide probes for every gene in the yeast genome.