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. 1998 Mar 31;95(7):3752–3757. doi: 10.1073/pnas.95.7.3752

Figure 2.

Figure 2

Fluorescence images of a high-density oligonucleotide array containing 25-mer probes for nearly every gene on Saccharomyces cerevisiae chromosomes 5–10. (a) Fluorescence pattern obtained after hybridization of 11 control genes: YEL002c, YEL003w, YEL005c, YEL006w, YEL018w, YEL019c, YEL021w, YEL024w, YHL014c, YHL045w, and YHL044c. Dark areas correspond to probes for genes not present in the control pool. (b) A close-up view of gene YHL014c shows the exact probe features that hybridize to the insert. Red grid highlights all probe features for YHL014c. Top row of probe elements contains oligonucleotides perfectly complementary to gene sequence, whereas bottom rows contain a mismatch in the central position of the oligonucleotide. Approximate locations of complementary oligonucleotide probes along the YHL014c ORF are also shown.