Table 1.
Yeast ORFs identified by array analysis of two-hybrid screens
| Clone type | YMR117c | YMR138w (CIN4) |
|---|---|---|
| Genes | YBR020w | YDL117w |
| YCL032W (STE50) | YDR087c | |
| YCR073c (SSK22) | YGL172w (NUP49) | |
| YDR104c | YHR141c (MAK18) | |
| YER018c | YLR109w | |
| YER032w (FIR1) | YNR050c (LYS9) | |
| YFR046c | YPL241c (CIN2) | |
| YGL197w | ||
| YIL144w | ||
| YLR319c (BUD6) | ||
| YLR419w | ||
| YML049c | ||
| YMR224c (MRE11) | ||
| YOL18c | ||
| YOL34w | ||
| YOR206w | ||
| YPR010c (RPA135) | ||
| YPR145w (ASN1) | ||
| DNA not encoding protein | 18S and 25S rRNA | |
| Reverse orientation | YNL291c | YBR189w |
| YDR381w | ||
| YNL301c (RP28B) | ||
| YNR035c | ||
| YOL056w (GPM3) |
ORF loci and names are listed for genes detected by array hybridization of PCR products derived from end products of a two-hybrid screen. Because inserts in the noncoding orientation comprise a significant proportion of false positives in the two-hybrid screen, RNA was transcribed from the upstream T7 promoter and used to generate exclusively antisense cDNA strands with reverse transcriptase. cDNA products were then biotinylated, fragmented, and hybridized as described. Genes detected by a double-stranded DNA hybridization but absent in cDNA hybridization are considered to be in reverse orientation. Control experiments were performed to confirm that this method is orientation-specific (data not shown).