Table 2.
Comparison of sequencing and hybridization for clone 5′ ends
| ORF name | ORF size, nt | 5′ end by sequencing | 5′ end array probe |
|---|---|---|---|
| YBR020w | 1,584 | 1,151 | 1,164 |
| YCL032w | 1,038 | 131 | 168 |
| YDR104c | 3,735 | 3,230 | 3,234 |
| YER032w | 2,775 | 1,808 | 1,860 |
| YFR046c | 1,083 | 4 | 114 |
| YGL197w | 4,461 | 3,974 | 4,092 |
| YML049c | 4,083 | 2,597 | 2,616 |
| YMR224c | 2,076 | 531 | 566 |
| YOL018c | 1,191 | 257 | 324 |
| YOL034w | 3,279 | 620 | 669 |
ORF name, ORF size, and the 5′ ends of identified genes, determined either by sequencing or array hybridization, for 10 clones from the YMR117c screen. For genes sequenced multiple times as different inserts, the end of the most 5′ clone is listed. The 5′ end as detected by array hybridization indicates the most 5′ nucleotide of the most 5′ probe detected as positive. Small disparities between sequencing and hybridization are the result of insert 5′ ends falling in between probes on the array. Although array hybridization does not confirm the inserts are in-frame with respect to the start codon, previous work has shown that frameshifting events generally lead to production of protein regardless of the precise fusion junction between gene insert and transcriptional activation domain (4).