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. 1998 Mar 31;95(7):3758–3763. doi: 10.1073/pnas.95.7.3758

Figure 3.

Figure 3

Expression of USF in mutant embryos. (A) A pregnancy resulting from the mating of Usf1(+/−) mice was interrupted at day 13.5 postconception, and MEF cell lines were established from each embryo. Genomic DNA was prepared from each cell line and used to establish the genotype of each embryo by Southern blotting. Mini nuclear extracts were prepared from each cell line and analyzed for USF DNA-binding species by EMSA, using a radiolabeled DNA fragment containing a USF-specific binding site under conditions that allow maximum resolution of the different USF dimers. Migration of protein–DNA complexes containing USF1 and USF2 polypeptides is indicated at left. (B) Same as in A but with embryos produced by Usf2(+/−) mice. (C) Nuclear extracts from MEFs of the indicated genotypes were analyzed by Western blotting using successively USF2- and USF1-specific antibodies. The asterisk indicates the migration of a USF-unrelated protein that interacted with the USF1 antibodies and represents an internal marker for equal protein loading in the different lanes.