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. 1998 Mar 31;95(7):3770–3775. doi: 10.1073/pnas.95.7.3770

Figure 3.

Figure 3

Diagram of cloned D. yakuba DNA fragments (bars HindIII, EcoRI, and PCR) showing their relation to the deduced organization of the HeT-A elements from which they were derived. The deduced head-to-tail elements are diagrammed below the three cloned fragments. 5′ and 3′ noncoding regions are striped and coding regions are open. Arrowheads at 3′ ends represent oligo(A) sequences. The double arrowhead between the two elements indicates the 25-bp 3′ end at the junction. The two dark arrows below the EcoRI clone indicate the location and orientation of PCR primers used to amplify the segment between the two EcoRI sites. The bar labeled PCR indicates only the new sequence in the PCR fragment and not the sequence that overlaps the two ends of the EcoRI clone. The PCR bar is arbitrarily placed below the right end of the EcoRI clone although it could have come from either end. S, SmaI; A, AlfIII; N, NdeI; H, HindIII; E, EcoRI.