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. 1998 Mar 31;95(7):3770–3775. doi: 10.1073/pnas.95.7.3770

Figure 4.

Figure 4

Southern blot showing that HeT-Ayak probes give strong hybridization to DNA from D. yakuba and D. teissieri but very little to DNA from more distantly related species. EcoRI-digested DNA from the species shown in Fig. 2 was probed with 32P-labeled coding sequence from HeT-Ayak. A long exposure is shown because faint hybridization to DNA from D. melanogaster and D. simulans can be detected. The final wash was 1× SSC at 65°C.