Table 2.

Determination of the affinities of the Fabs examined here using the direct calibration ELISA method. The three components of the analysis are given, as well as the affinity calculated as the dissociation constant. We were unable to accurately determine the affinity of Fab B using this method. ND = not determined.

Antibody ID	Rate of complex formation (Kc, s−1)	Calibration Factor (c, M)	Transfer Factor (F)	Dissociation constant (Kd, M)
14	1.2 × 10−3	7.0 × 10−10	0.9	3.4 × 10−9
15	3.4 × 10−3	1.3 × 10−9	0.7	2.1 × 10−9
16	1.0 × 10−3	8.6 × 10−10	0.83	2.6 × 10−9
6	1.3 × 10−3	4.8 × 10−11	0.99	3.2 × 10−9
8	1.7 × 10−3	1 × 10−9	0.82	3.4 × 10−9
B	ND	ND	ND	~6.1 × 10−9
F	1.7 × 10−3	8 × 10−9	0.39	1.5 × 10−9
E	8.5 × 10−4	2 × 10−9	0.8	1.7 × 10−9