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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biomaterials. 2007 Jan 26;28(12):2097–2108. doi: 10.1016/j.biomaterials.2007.01.008

Fig. 2.

Fig. 2

Neurite growth is greater in salmon fibrin than in mammalian fibrin or Matrigel. A, Mouse spinal cord neurons embedded in fibrin for 2 days (left 3 panels) or 3 days (right two panels) were immunostained with markers of neurites (TuJ1) or axons (NFH, phosphorylated neurofilament H). Scale bar is 50 μm. B, Rat cortical neurons on laminin-coated glass coverslips or embedded in fibrin for 3 days were immunostained with TuJ1 or Map5/Map1b to visualize neurites. The long neurite in the salmon fibrin has been highlighted to distinguish it from the other neurites in the field (see Supplemental data for the non-highlighted image). Scale bar is 50 μm. C, The effect of the different matrices on neurite growth was quantified by measuring the total neurite length per cell (**p<0.001 salmon fibrin vs. bovine fibrin, human fibrin, or Matrigel, error bars represent s.e.m.).