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Single turnover binding of AP-site containing DNA by AP endonuclease mutants Y128A (●), Y269A (◆) and Y171A (□). For the experiment shown in the figure, substrate and enzyme were each present at 4 nM for Y128A, at 4 nM and 0.4 nM respectively for Y269A and at 7 nM for Y171A. [ES]eq for Y128A was 0.28 nM, when initial concentration of enzyme and substrate were each 4 nM. ([ES]eq for Y269A was 0.1 nM when initial enzyme and substrate were 0.4 nM and 4 nM respectively).
