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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jan 10;6(3):374–382. doi: 10.1016/j.dnarep.2006.11.004

Figure 4.

Figure 4

Figure 4

Comparison of binding AP-site containing DNA with (●, ◆) and without (○) an AP-site by EMSA analysis. WT and mutant proteins at the indicated concentrations were incubated with 0.25 nM substrate and resolved by non-denaturing gel electrophoresis as described in Methods. The distribution of bound and unbound substrate was quantitated by phosphorImager analysis.

A: Binding AP-site-containing DNA by Y128A(●) and Y269A (◆)

B: Binding by WT AP endo or Tyr171 mutants to oligonucleotide with (●) or without(○) an abasic site.