Figure 1.
Evaluation of enrichment for (CA)n-containing fragments after affinity capture. We show an ethidium bromide-stained agarose gel (A) and a Southen blot hybridized with an alkaline phosphatase-labeled (CA)8 probe (B). Lanes: 1, T. cruzi DNA digested with Sau3AI; 2, T. cruzi DNA digested and ligated with Sau3AI adapters; and 3, T. cruzi DNA digested with Sau3AI with adapters after capturing the (CA)n-containing fragments. Molecular size markers were from a 1-Kb ladder (Life Technologies, Gaithersburg, MD) are indicated.