Figure 3.

Unrooted Wagner network of 20 T. cruzi strains based on the amplification of eight polymorphic loci with (CA)_n repeats. Considering each microsatellite allele as one state from a multistate character, the genetic distance between any two strains was estimated as the number of mutational steps necessary to transform one into the other. The figures in parenthesis indicate the number of times that the branch was observed in 1,000 bootstraps. Bootstrap values inside the lower cluster (below strain 138) were all low (below 300) and are not given individually. The classification of each strain indicated in italics (1, 2, or 1/2) was based on the rRNA 24Sα amplification.