Table 2.
Microsatellites markers isolated from T. cruzi
| Marker | Size range alleles, bp | Repeat sequence | PCR primers 5′ → 3′ | Number of alleles found | Observed heterozygosity | Expected heterozygosity |
|---|---|---|---|---|---|---|
| MCLE01 | 110–150 | (CA)9 | 1 CTGCCATGTTTGATCCCT 2 CGTGTACATATCGGCAGTG | 11 | 0.60 | 0.84 |
| MCLE08 | 124–130 | (CA)2AA(CA)12 | 1 ATGGACAACAAATGGGAG 2 TGGGTATGCCAAATGTGAT | 4 | 0.20 | 0.71 |
| SCLE10 | 237–291 | (GT)2(TG)10 | 1 GATCCCGCAATAGGAAAC 2 GTGCATGTTCCATGGCTT | 12 | 0.80 | 0.87 |
| SCLE11 | 139–157 | (AC)9 | 1 ACGACCAAAGCCATCATT 2 GATGCTAACTGCTCAAGTGA | 9 | 0.60 | 0.83 |
| MCLF10 | 182–194 | (CA)2A(CA)14 | 1 GCGTAGCGATTCATTTCC 2 ATCCGCTACCACTATCCAC | 5 | 0.35 | 0.53 |
| MCLG10 | 151–187 | (CA)8 | 1 AGGAGTCAAATATAATGAGGCA 2 ACGTGTGAAAGGCATCTATC | 7 | 0.20 | 0.69 |
| MCL03 | 257–319 | (CT)4(GT)2CT AT(GT)15 | 1 GGAGCAAGAATGAAGGCA 2 TCAGAAAAAGCACGCCTC | 13 | 0.65 | 0.91 |
| MCL05 | 194–228 | (TC)9(GT)4 | 1 TTAAACGACCTCTATGTCTCTC 2 CCTGAGCAAGATACAAGGAC | 12 | 0.55 | 0.90 |